5SYE
Near-atomic resolution cryo-EM reconstruction of doubly bound Taxol- and peloruside-stabilized microtubule
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | HELICAL |
Resolution (Å) | 3.5 |
Number of Particle | 27275 |
Software | EMAN2,FREALIGN,Rosetta,REFMAC,Leginon,Appion,CTFFIND,UCSF Chimera |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |