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5SYE

Near-atomic resolution cryo-EM reconstruction of doubly bound Taxol- and peloruside-stabilized microtubule

EM specimen
VitrificationYes
InstrumentFEI VITROBOT MARK IV
EM Data Collection
MicroscopeFEI TITAN
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
Cs (mm)2.7
DetectorGATAN K2 SUMMIT (4k x 4k)
3D Reconstruction
MethodHELICAL
Resolution (Å)3.5
Number of Particle27275
SoftwareEMAN2,FREALIGN,Rosetta,REFMAC,Leginon,Appion,CTFFIND,UCSF Chimera
CTF correctionPHASE FLIPPING AND AMPLITUDE CORRECTION

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PDB entries from 2024-11-06

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