5SY5
Crystal Structure of the Heterodimeric NPAS1-ARNT Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-24 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 69.948, 81.187, 138.062 |
Unit cell angles | 90.38, 95.08, 107.39 |
Refinement procedure
Resolution | 38.926 - 3.201 |
R-factor | 0.18 |
Rwork | 0.176 |
R-free | 0.24670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zp4 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.361 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 8.670 | 3.200 |
Rmerge | 0.055 | 0.026 | 0.764 |
Number of reflections | 46508 | ||
<I/σ(I)> | 9.5 | ||
Completeness [%] | 98.6 | 94.6 | 98.3 |
Redundancy | 2.1 | 2.1 | 2.2 |
CC(1/2) | 0.993 | 0.459 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 2% Tacsimate pH 7.0, 3% PEG3350 |