5SWQ
Crystal Structure of HLA-A*0201 in complex with NA231, an influenza epitope
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-30 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.450, 80.060, 55.000 |
| Unit cell angles | 90.00, 111.36, 90.00 |
Refinement procedure
| Resolution | 19.788 - 2.000 |
| R-factor | 0.1974 |
| Rwork | 0.194 |
| R-free | 0.25830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gso |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.165 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.100 | |
| High resolution limit [Å] | 2.000 | 10.000 | 2.000 |
| Rmerge | 0.105 | 0.035 | 0.437 |
| Number of reflections | 27785 | ||
| <I/σ(I)> | 10.57 | 36.43 | 3.15 |
| Completeness [%] | 98.6 | 78.5 | 99.4 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 20%PEG6K, 0.1M Na-Citrate pH6.5, 0.2M NaCl |
