5SW2
Thaumatin Structure at pH 6.0, orthorhombic type1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-18 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.8 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.786, 53.861, 70.746 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.900 - 1.200 |
R-factor | 0.1379 |
Rwork | 0.137 |
R-free | 0.16480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vhg |
RMSD bond length | 0.006 |
RMSD bond angle | 1.126 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.900 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.064 | 0.700 |
Number of reflections | 61408 | |
<I/σ(I)> | 18 | 2.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.8 | 7.2 |
CC(1/2) | 0.999 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG4000, 50 mM ADA |