5SVT
Anomalous Cs+ signal reveals the site of Na+ ion entry to the channel pore of the human P2X3 ion channel through the extracellular fenestrations
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-11 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.90740 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 119.950, 119.950, 236.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.725 - 3.794 |
| R-factor | 0.278 |
| Rwork | 0.276 |
| R-free | 0.31190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.769 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2142: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.790 |
| Number of reflections | 12566 |
| <I/σ(I)> | 13.42 |
| Completeness [%] | 99.8 |
| Redundancy | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 25% PEG 400, 100 mM MES, pH 6.85, 50 mM MgCl2, 100 mM CsCl |
