5SVL

Crystal structure of the ATP-gated human P2X3 ion channel in the ATP-bound, closed (desensitized) state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979
Spacegroup name	P 21 3
Unit cell lengths	172.140, 172.140, 172.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.983 - 2.900
R-factor	0.2069
Rwork	0.205
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.350
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (dev_1634)

Data quality characteristics

	Overall
Low resolution limit [Å]	80.000
High resolution limit [Å]	2.900
Number of reflections	62472
<I/σ(I)>	12.3
Completeness [%]	97.6
Redundancy	2.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	21% PEG 400, 100 mM Tris, pH 8.0, 325 mM Sodium Acetate, 100 mM NaCl