5SVL
Crystal structure of the ATP-gated human P2X3 ion channel in the ATP-bound, closed (desensitized) state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-11-15 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 3 |
Unit cell lengths | 172.140, 172.140, 172.140 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.983 - 2.900 |
R-factor | 0.2069 |
Rwork | 0.205 |
R-free | 0.23980 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.350 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1634) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 80.000 |
High resolution limit [Å] | 2.900 |
Number of reflections | 62472 |
<I/σ(I)> | 12.3 |
Completeness [%] | 97.6 |
Redundancy | 2.84 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 21% PEG 400, 100 mM Tris, pH 8.0, 325 mM Sodium Acetate, 100 mM NaCl |