5SVK

Crystal structure of the ATP-gated human P2X3 ion channel in the ATP-bound, open state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-11
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 3
Unit cell lengths	173.150, 173.150, 173.150
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.287 - 2.773
R-factor	0.2019
Rwork	0.201
R-free	0.22770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.892
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.770
Number of reflections	79845
<I/σ(I)>	12.34
Completeness [%]	99.2
Redundancy	3.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	20% PEG 400, 50 mM ADA, pH 6.5