5SVH

Crystal structure of the KIX domain of CBP in complex with a MLL/c-Myb chimera

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-14
Detector	MAR CCD 165 mm
Wavelength(s)	1.0332
Spacegroup name	P 63 2 2
Unit cell lengths	119.820, 119.820, 50.430
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.900 - 2.050
R-factor	0.2082
Rwork	0.206
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2agh
RMSD bond length	0.007
RMSD bond angle	0.970
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.900	2.123
High resolution limit [Å]	2.050	2.050
Rmerge	0.066	0.959
Number of reflections	13648
<I/σ(I)>	31	3.8
Completeness [%]	98.7	96.5
Redundancy	27.1	24.7
CC(1/2)		0.871

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M potassium sulfate 2.3M Ammonium sulphate