5SV7
The Crystal structure of a chaperone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 32 |
| Unit cell lengths | 163.913, 163.913, 63.076 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.851 - 3.209 |
| R-factor | 0.2732 |
| Rwork | 0.271 |
| R-free | 0.32180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4yzs |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.699 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 37.851 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 30419 |
| <I/σ(I)> | 40.4 |
| Completeness [%] | 99.0 |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | MES 0.1M, PEG 3350 |
