5SUZ

Domain-swapped dimer of human Dishevelled2 DEP domain: C-centered monoclinic crystal form crystallised from monomeric fraction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-17
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.928190
Spacegroup name	C 1 2 1
Unit cell lengths	117.785, 59.601, 30.322
Unit cell angles	90.00, 93.64, 90.00

Refinement procedure

Resolution	32.700 - 1.840
R-factor	0.2195
Rwork	0.217
R-free	0.26270
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.555
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.700	1.910
High resolution limit [Å]	1.840	1.840
Rmerge	0.107	2.416
Number of reflections	17446
<I/σ(I)>	16.1	0.98
Completeness [%]	92.0
Redundancy	13.7	13.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1 M MES, pH=6.5 0.2 M CaCl2 23% PEG350 MME