5SBB
Tubulin-maytansinoid-4c-complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-23 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00003 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.615, 157.484, 180.633 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.540 - 2.250 |
R-factor | 0.1854 |
Rwork | 0.183 |
R-free | 0.22750 |
Structure solution method | DIFFERENCE FOURIER |
Starting model (for MR) | 5lxt |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.19.1_4122) |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.540 | 49.540 | 2.310 |
High resolution limit [Å] | 2.250 | 10.060 | 2.250 |
Rmerge | 0.189 | 0.042 | 2.489 |
Rmeas | 0.196 | 0.044 | 2.591 |
Total number of observations | 1937774 | ||
Number of reflections | 141155 | 1738 | 10223 |
<I/σ(I)> | 11.75 | 50.44 | 0.99 |
Completeness [%] | 99.3 | 98.7 | 98.5 |
Redundancy | 13.728 | 12.758 | 13.077 |
CC(1/2) | 0.998 | 0.999 | 0.408 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 3% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |