5SB6

Tubulin-todalam-10-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	104.270, 156.726, 180.456
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.470 - 2.300
R-factor	0.2023
Rwork	0.201
R-free	0.23660
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.19.1_4122)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.470	49.470	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.228	0.030	3.662
Rmeas	0.237	0.031	3.822
Total number of observations	1761238
Number of reflections	131228	1620	9549
<I/σ(I)>	11.79	70.35	0.64
Completeness [%]	99.9	98.8	99.4
Redundancy	13.421	13.051	11.963
CC(1/2)	0.998	1.000	0.206

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine