5S61

Tubulin-Z57472297-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.360, 159.310, 178.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.590 - 1.950
R-factor	0.215
Rwork	0.214
R-free	0.23930
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.590	62.590	2.000
High resolution limit [Å]	1.950	8.720	1.950
Rmerge	0.073	0.023	2.188
Rmeas	0.079	0.025	2.433
Total number of observations	1408241
Number of reflections	217751	2676	15916
<I/σ(I)>	13.01	58.76	0.7
Completeness [%]	99.9	99.6	99.8
Redundancy	6.467	6.12	5.341
CC(1/2)	0.999	0.999	0.262

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine