5S5U
Tubulin-Z1124201124-complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.780, 158.650, 178.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.330 - 2.500 |
R-factor | 0.2121 |
Rwork | 0.210 |
R-free | 0.25580 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 79.330 | 79.330 | 2.560 |
High resolution limit [Å] | 2.500 | 11.180 | 2.500 |
Rmerge | 0.110 | 0.030 | 2.746 |
Rmeas | 0.119 | 0.033 | 2.971 |
Total number of observations | 700397 | ||
Number of reflections | 103364 | 1289 | 7557 |
<I/σ(I)> | 10.67 | 52.72 | 0.64 |
Completeness [%] | 99.8 | 98.8 | 99.6 |
Redundancy | 6.776 | 6.335 | 6.828 |
CC(1/2) | 0.998 | 0.998 | 0.271 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |