5S5T

Tubulin-Z198194394-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.834, 158.225, 178.442
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.320 - 2.530
R-factor	0.2119
Rwork	0.211
R-free	0.25560
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.320	72.320	2.600
High resolution limit [Å]	2.530	11.310	2.530
Rmerge	0.098	0.041	1.769
Rmeas	0.106	0.045	1.911
Total number of observations	681711
Number of reflections	100954	1263	7391
<I/σ(I)>	11.39	38.02	1.18
Completeness [%]	100.0	99.2	100
Redundancy	6.753	6.235	6.958
CC(1/2)	0.998	0.998	0.507

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine