5S5S

Tubulin-Z166605480-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.550, 158.051, 179.339
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.320 - 2.360
R-factor	0.2124
Rwork	0.212
R-free	0.25350
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.320	72.320	2.420
High resolution limit [Å]	2.360	10.550	2.360
Rmerge	0.121	0.053	2.026
Rmeas	0.131	0.058	2.187
Total number of observations	848024
Number of reflections	124208	1538	9105
<I/σ(I)>	8.81	30.03	0.86
Completeness [%]	99.9	99.3	99.9
Redundancy	6.827	6.259	7.064
CC(1/2)	0.997	0.996	0.451

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine