5S5Q
Tubulin-Z396380540-complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.850, 158.620, 178.620 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 90.420 - 2.050 |
R-factor | 0.2136 |
Rwork | 0.212 |
R-free | 0.24400 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 90.420 | 90.420 | 2.100 |
High resolution limit [Å] | 2.050 | 9.170 | 2.050 |
Rmerge | 0.081 | 0.031 | 2.134 |
Rmeas | 0.088 | 0.033 | 2.352 |
Total number of observations | 1239010 | ||
Number of reflections | 186150 | 2294 | 13655 |
<I/σ(I)> | 11.63 | 48.17 | 0.76 |
Completeness [%] | 99.8 | 99.4 | 99.7 |
Redundancy | 6.656 | 6.081 | 5.728 |
CC(1/2) | 0.999 | 0.999 | 0.334 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |