5S5N
Tubulin-Z165170770-complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.610, 159.430, 179.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.620 - 2.900 |
R-factor | 0.2245 |
Rwork | 0.222 |
R-free | 0.27350 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 63.620 | 63.620 | 2.980 |
High resolution limit [Å] | 2.900 | 12.970 | 2.900 |
Rmerge | 0.149 | 0.021 | 2.517 |
Rmeas | 0.162 | 0.022 | 2.718 |
Total number of observations | 441656 | ||
Number of reflections | 66075 | 861 | 4951 |
<I/σ(I)> | 9.98 | 59.92 | 0.84 |
Completeness [%] | 97.4 | 99 | 99.9 |
Redundancy | 6.684 | 6.092 | 7.036 |
CC(1/2) | 0.999 | 1.000 | 0.491 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |