5S5I

Tubulin-Z295848548-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.890, 158.870, 179.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.660 - 2.490
R-factor	0.2146
Rwork	0.212
R-free	0.25870
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.660	62.660	2.550
High resolution limit [Å]	2.490	11.140	2.490
Rmerge	0.134	0.026	2.706
Rmeas	0.145	0.029	2.929
Total number of observations	689436
Number of reflections	103520	1319	7681
<I/σ(I)>	9.9	52.58	0.67
Completeness [%]	98.2	99.1	99.7
Redundancy	6.66	6.372	6.839
CC(1/2)	0.998	0.999	0.262

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine