5S5F
Tubulin-Z87615031-complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.828, 158.374, 179.214 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 72.430 - 2.240 |
R-factor | 0.2359 |
Rwork | 0.236 |
R-free | 0.26170 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 72.430 | 72.430 | 2.300 |
High resolution limit [Å] | 2.240 | 10.020 | 2.240 |
Rmerge | 0.136 | 0.079 | 1.932 |
Rmeas | 0.148 | 0.086 | 2.110 |
Total number of observations | 981011 | ||
Number of reflections | 144973 | 1787 | 10540 |
<I/σ(I)> | 7.58 | 21.42 | 0.87 |
Completeness [%] | 99.9 | 99.3 | 99.5 |
Redundancy | 6.767 | 6.193 | 6.235 |
CC(1/2) | 0.994 | 0.993 | 0.591 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |