5S5C
Tubulin-Z44592329-complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.220, 159.900, 177.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 67.930 - 2.400 |
R-factor | 0.2091 |
Rwork | 0.207 |
R-free | 0.24390 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 67.930 | 67.930 | 2.460 |
High resolution limit [Å] | 2.400 | 10.730 | 2.400 |
Rmerge | 0.116 | 0.027 | 2.277 |
Rmeas | 0.126 | 0.029 | 2.460 |
Total number of observations | 797912 | ||
Number of reflections | 117490 | 1460 | 8604 |
<I/σ(I)> | 10.22 | 51.45 | 0.82 |
Completeness [%] | 99.8 | 99.1 | 99.8 |
Redundancy | 6.791 | 6.373 | 6.99 |
CC(1/2) | 0.999 | 0.999 | 0.383 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |