5S5B
Tubulin-Z906021418-complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.020, 158.920, 179.410 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 63.370 - 2.300 |
R-factor | 0.211 |
Rwork | 0.209 |
R-free | 0.24380 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 63.370 | 63.370 | 2.360 |
High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
Rmerge | 0.111 | 0.031 | 2.332 |
Rmeas | 0.120 | 0.033 | 2.515 |
Total number of observations | 908247 | ||
Number of reflections | 133439 | 1657 | 9775 |
<I/σ(I)> | 9.62 | 45.49 | 0.74 |
Completeness [%] | 99.9 | 99.3 | 99.8 |
Redundancy | 6.806 | 6.302 | 7.068 |
CC(1/2) | 0.999 | 0.999 | 0.326 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |