5S59

Tubulin-Z1324080698-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.560, 158.900, 179.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.840 - 2.600
R-factor	0.222
Rwork	0.220
R-free	0.26810
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.840	59.840	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.106	0.024	3.029
Rmeas	0.115	0.026	3.300
Total number of observations	622674
Number of reflections	92582	1161	6770
<I/σ(I)>	12.14	61.56	0.58
Completeness [%]	99.3	98.4	99.4
Redundancy	6.726	6.411	6.392
CC(1/2)	0.999	0.999	0.305

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine