5S54

Tubulin-Z2856434816-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.330, 157.150, 179.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.960 - 2.400
R-factor	0.208
Rwork	0.206
R-free	0.25040
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.960	71.960	2.460
High resolution limit [Å]	2.400	10.730	2.400
Rmerge	0.095	0.036	2.237
Rmeas	0.103	0.039	2.414
Total number of observations	784868
Number of reflections	115397	1446	8423
<I/σ(I)>	10.58	41.42	0.72
Completeness [%]	99.9	99.4	99.7
Redundancy	6.801	6.322	7.017
CC(1/2)	0.999	0.999	0.385

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine