5S53
Tubulin-Z1349163663-complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 105.320, 159.310, 178.960 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.190 - 2.750 |
R-factor | 0.22 |
Rwork | 0.218 |
R-free | 0.26690 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 68.190 | 68.190 | 2.820 |
High resolution limit [Å] | 2.750 | 12.300 | 2.750 |
Rmerge | 0.126 | 0.019 | 2.542 |
Rmeas | 0.137 | 0.021 | 2.750 |
Total number of observations | 524219 | ||
Number of reflections | 77724 | 990 | 5699 |
<I/σ(I)> | 11.24 | 65.17 | 0.79 |
Completeness [%] | 98.5 | 98.6 | 98.7 |
Redundancy | 6.745 | 6.273 | 6.89 |
CC(1/2) | 0.999 | 1.000 | 0.466 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |