5S50

Tubulin-Z57299526-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.030, 158.020, 180.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.010 - 3.100
R-factor	0.2107
Rwork	0.208
R-free	0.26970
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	79.010	79.010	3.180
High resolution limit [Å]	3.100	13.860	3.100
Rmerge	0.088	0.019	2.273
Rmeas	0.096	0.021	2.456
Total number of observations	367126
Number of reflections	54710	689	3976
<I/σ(I)>	14.78	73.42	0.84
Completeness [%]	99.8	97.5	99.7
Redundancy	6.71	6.206	6.956
CC(1/2)	0.999	0.999	0.405

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine