5S4Y

Tubulin-Z2856434857-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.140, 157.780, 178.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.890 - 2.300
R-factor	0.2022
Rwork	0.200
R-free	0.23770
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	78.890	78.890	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.080	0.040	2.664
Rmeas	0.087	0.043	2.873
Total number of observations	893148
Number of reflections	130903	1631	9582
<I/σ(I)>	11.23	40.3	0.66
Completeness [%]	99.9	99.5	99.9
Redundancy	6.823	6.272	7.081
CC(1/2)	0.999	0.998	0.277

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine