5S4X

Tubulin-Z2856434917-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.110, 159.220, 180.520
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.840 - 2.530
R-factor	0.204
Rwork	0.202
R-free	0.24480
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.840	72.840	2.600
High resolution limit [Å]	2.530	11.310	2.530
Rmerge	0.085	0.023	2.466
Rmeas	0.092	0.025	2.672
Total number of observations	678312
Number of reflections	100799	1263	7427
<I/σ(I)>	13.52	62.37	0.78
Completeness [%]	99.2	98.7	100
Redundancy	6.729	6.364	6.772
CC(1/2)	0.999	1.000	0.320

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine