5S4R

Tubulin-Z117233350-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.360, 159.890, 180.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.920 - 2.350
R-factor	0.2153
Rwork	0.213
R-free	0.25090
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.920	62.920	2.410
High resolution limit [Å]	2.350	10.510	2.350
Rmerge	0.109	0.027	3.527
Rmeas	0.119	0.029	3.809
Total number of observations	842941
Number of reflections	126292	1564	9165
<I/σ(I)>	9.55	48.45	0.6
Completeness [%]	99.5	99	99
Redundancy	6.675	6.226	7
CC(1/2)	0.999	0.999	0.256

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine