5S4O
Tubulin-Z48847594-complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.91587 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.744, 158.001, 178.586 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 79.000 - 2.300 |
R-factor | 0.2164 |
Rwork | 0.216 |
R-free | 0.26170 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX (1.18.2_3874) |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 79.000 | 79.000 | 2.360 |
High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
Rmerge | 0.133 | 0.072 | 1.731 |
Rmeas | 0.144 | 0.079 | 1.878 |
Total number of observations | 905186 | ||
Number of reflections | 133616 | 1661 | 9784 |
<I/σ(I)> | 7.51 | 20.76 | 1.01 |
Completeness [%] | 100.0 | 99.3 | 100 |
Redundancy | 6.775 | 6.128 | 6.647 |
CC(1/2) | 0.995 | 0.995 | 0.473 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine |