5S4M

Tubulin-Z2142244288-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	104.670, 157.870, 179.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.020 - 2.150
R-factor	0.2079
Rwork	0.206
R-free	0.24350
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.020	63.020	2.210
High resolution limit [Å]	2.150	9.620	2.150
Rmerge	0.104	0.037	2.842
Rmeas	0.113	0.040	3.099
Total number of observations	1075851
Number of reflections	159163	1992	11800
<I/σ(I)>	9.48	39.87	0.58
Completeness [%]	98.6	99.6	99.6
Redundancy	6.759	6.212	6.305
CC(1/2)	0.998	0.998	0.238

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine