5S4L

Tubulin-Z1891773393-complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.91587
Spacegroup name	P 21 21 21
Unit cell lengths	105.370, 159.780, 178.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	63.660 - 2.300
R-factor	0.2037
Rwork	0.202
R-free	0.23460
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	5lxt
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	63.660	63.660	2.360
High resolution limit [Å]	2.300	10.290	2.300
Rmerge	0.077	0.020	1.693
Rmeas	0.083	0.022	1.825
Total number of observations	911118
Number of reflections	133828	1671	9789
<I/σ(I)>	14.88	68.73	1.12
Completeness [%]	99.6	99.2	99.3
Redundancy	6.808	6.305	7.093
CC(1/2)	0.999	1.000	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2% PEG 4K, 4% glycerol, 30 mM MgCl2, 30 mM CaCl2, 0.1 M MES/Imidazole, 5 mM L-tyrosine