5QCH
Crystal structure of human Cathepsin-S with bound ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-10 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.120, 68.130, 79.020 |
| Unit cell angles | 88.06, 87.69, 73.08 |
Refinement procedure
| Resolution | 34.110 - 2.200 |
| R-factor | 0.201 |
| Rwork | 0.199 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.990 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.110 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 51559 |
| Completeness [%] | 94.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 100MM SODIUM ACETATE PH 4.5, 200MM AMMONIUM ACETATE, 25% PEG 8000. PROTEIN CONCENTRATION 7 MG/ML, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
