5QC9
Crystal structure of human Cathepsin-S with bound ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-10 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.088, 57.914, 59.044 |
| Unit cell angles | 102.44, 107.47, 106.22 |
Refinement procedure
| Resolution | 32.470 - 2.000 |
| R-factor | 0.138 |
| Rwork | 0.136 |
| R-free | 0.16900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.804 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.468 |
| High resolution limit [Å] | 1.999 |
| Number of reflections | 27173 |
| Completeness [%] | 94.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 100MM SODIUM ACETATE PH 4.5, 200MM AMMONIUM ACETATE, 25% PEG 8000. PROTEIN CONCENTRATION 7 MG/ML, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
