5Q0Q
Ligand binding to FARNESOID-X-RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.439, 131.892, 37.409 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.410 - 2.600 |
| R-factor | 0.248 |
| Rwork | 0.246 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.010 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.700 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.162 | 0.640 |
| Number of reflections | 16123 | 1520 |
| <I/σ(I)> | 7.68 | 1.26 |
| Completeness [%] | 98.4 | 88.1 |
| Redundancy | 5.34 | 2.81 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | evaporation, hanging drop | 7.5 | 298 | 0.1 M HEPES pH 7.5, 2.0 M Ammonium Sulfate |
