5PNT
CRYSTAL STRUCTURE OF A HUMAN LOW MOLECULAR WEIGHT PHOSPHOTYROSYL PHOSPHATASE. IMPLICATIONS FOR SUBSTRATE SPECIFICITY
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-02 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.100, 56.600, 97.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.200 |
| R-factor | 0.181 |
| Rwork | 0.181 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pnt |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.018 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.077 | 0.245 |
| Total number of observations | 102917 * | |
| Number of reflections | 9356 | |
| <I/σ(I)> | 10 | 10 |
| Completeness [%] | 92.2 | 64 |
| Redundancy | 10 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | mPEG5000 | 30 (%) | |
| 3 | 1 | drop | ammonium sulfate | 200 (mM) | |
| 4 | 1 | drop | MES | 100 (mM) | pH6.5 |
