5PGM

SACCHAROMYCES CEREVISIAE PHOSPHOGLYCERATE MUTASE

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-09
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	82.497, 93.262, 147.329
Unit cell angles	90.00, 90.15, 90.00

Refinement procedure

Resolution	30.000 - 2.120
R-factor	0.197 *
Rwork	0.196
R-free	0.22900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pgm
RMSD bond length	0.016
RMSD bond angle	24.210 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.240
High resolution limit [Å]	2.120	2.120
Rmerge	0.062	0.184 *
Number of reflections	120182
<I/σ(I)>	8.3	4
Completeness [%]	90.8 *	81.8 *
Redundancy	2.6	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8.65		Rigden, D.J., (1998) J.Mol.Biol., 276, 449. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	inositol hexakisphosphate	1 (mM)
3	1	reservoir	Tris-HCl	60 (mM)	pH8.65
4	1	reservoir	lithium sulfate	120 (mM)
5	1	reservoir	PEG4000	22-24 (%)