5OXS
Crystal structure of human lung surfactant protein D trimeric fragment with bound ligand Salmonella enterica Minnesota R5 oligosaccharide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-16 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.92819 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.679, 108.510, 56.170 |
Unit cell angles | 90.00, 92.89, 90.00 |
Refinement procedure
Resolution | 56.100 - 1.650 |
R-factor | 0.1658 |
Rwork | 0.165 |
R-free | 0.18680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 1PW9 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.324 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.12) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 56.100 | 56.100 | 1.680 |
High resolution limit [Å] | 1.650 | 9.040 | 1.650 |
Rmerge | 0.054 | 0.024 | 0.397 |
Rmeas | 0.065 | 0.029 | 0.480 |
Rpim | 0.036 | 0.016 | 0.266 |
Number of reflections | 74199 | 509 | 3629 |
<I/σ(I)> | 11.4 | ||
Completeness [%] | 93.0 | 99.2 | 93.2 |
Redundancy | 3.1 | 3.4 | 3 |
CC(1/2) | 0.998 | 0.998 | 0.694 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Tris pH 8.0, 16% PEG 6000 |