5OXH
C-terminally retracted ubiquitin T66V/L67N mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-03-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54179 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 49.770, 49.770, 89.270 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.885 - 1.601 |
| R-factor | 0.1931 |
| Rwork | 0.192 |
| R-free | 0.22290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq aa 1-59 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.073 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.885 | 1.658 |
| High resolution limit [Å] | 1.601 | 1.601 |
| Rmerge | 0.056 | 0.227 |
| Number of reflections | 17205 | 1675 |
| <I/σ(I)> | 22.8 | |
| Completeness [%] | 98.5 | 96.49 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | 3.2 M ammonium sulfate, 0.1 M bicine (pH 9.0); 1:1 protein to reservoir ratio |
