5OWE
Crystal structure of the human BRPF1 bromodomain in complex with DSPBP1010
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.000020 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 60.530, 60.530, 63.680 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.300 - 1.700 |
R-factor | 0.1927 |
Rwork | 0.190 |
R-free | 0.21340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4lc2 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.744 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.300 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.050 | 0.540 |
Number of reflections | 15062 | 794 |
<I/σ(I)> | 31.1 | |
Completeness [%] | 98.9 | 98.2 |
Redundancy | 19.8 | 20.9 |
CC(1/2) | 0.998 | 0.956 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Bis-tris propane, pH6.5, 0.2 M Sodium nitrate, 20% PEG3350 |