5OWB

Crystal structure of the human BRPF1 bromodomain in complex with DSPBP1004

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000020
Spacegroup name	P 32 2 1
Unit cell lengths	60.426, 60.426, 63.468
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.376 - 1.650
R-factor	0.1776
Rwork	0.175
R-free	0.20440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lc2
RMSD bond length	0.007
RMSD bond angle	0.831
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.380	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.067	0.627
Number of reflections	16528	815
<I/σ(I)>	26.7	4.3
Completeness [%]	99.8
Redundancy	19.3
CC(1/2)	0.999	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1M Sodium Acetate, pH5.5, 0.15 M Sodium Nitrate, 20% PEG3350