5OVT
Thiobacillus denitrificans BPH in complex with Epoxomicin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-21 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 196.820, 196.820, 296.370 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.200 - 2.950 |
R-factor | 0.17323 |
Rwork | 0.172 |
R-free | 0.18860 |
Structure solution method | SAD |
RMSD bond length | 0.020 |
RMSD bond angle | 2.111 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.200 | 3.120 |
High resolution limit [Å] | 2.950 | 2.950 |
Rmerge | 0.439 | 1.980 |
Number of reflections | 71632 | 10916 |
<I/σ(I)> | 13.6 | 3.11 |
Completeness [%] | 99.9 | 100 |
Redundancy | 57.1 | 57.6 |
CC(1/2) | 0.995 | 0.878 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 294 | 100 mM sodium acetate pH 4.8, 1.2 M sodium nitrate |