5OVL
crystal structure of MabA bound to NADP+ from M. smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-07 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.8729 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.140, 69.390, 223.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.256 - 2.400 |
| R-factor | 0.1713 |
| Rwork | 0.168 |
| R-free | 0.21940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uzn |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.404 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.260 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmeas | 0.166 | 0.520 |
| Number of reflections | 32703 | |
| <I/σ(I)> | 10.75 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Na citrate pH 5.6, 1.0 M Li2SO4 and 0.5 M (NH4)2SO4 |
