5OVJ
Structure of the apo form of Mycobacterium smegmatis MabA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 96.880, 68.270, 71.710 |
Unit cell angles | 90.00, 123.77, 90.00 |
Refinement procedure
Resolution | 34.135 - 1.700 |
R-factor | 0.162 |
Rwork | 0.160 |
R-free | 0.20160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ovl |
RMSD bond length | 0.006 |
RMSD bond angle | 0.731 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.135 | 1.761 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 42269 | 4171 |
<I/σ(I)> | 8.61 | |
Completeness [%] | 98.8 | 98.84 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | BIS TRIS, DMSO, ammonium sulfate |