5OVJ

Structure of the apo form of Mycobacterium smegmatis MabA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-11
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	96.880, 68.270, 71.710
Unit cell angles	90.00, 123.77, 90.00

Refinement procedure

Resolution	34.135 - 1.700
R-factor	0.162
Rwork	0.160
R-free	0.20160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ovl
RMSD bond length	0.006
RMSD bond angle	0.731
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.135	1.761
High resolution limit [Å]	1.700	1.700
Number of reflections	42269	4171
<I/σ(I)>	8.61
Completeness [%]	98.8	98.84
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	BIS TRIS, DMSO, ammonium sulfate