5OV3

Structure of the RbBP5 beta-propeller domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	77
Detector technology	PIXEL
Collection date	2015-08-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	56.809, 71.897, 178.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.678 - 2.450
R-factor	0.1995
Rwork	0.196
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xl2
RMSD bond length	0.009
RMSD bond angle	1.041
Data reduction software	XDS
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	71.900
High resolution limit [Å]	2.450
Number of reflections	27559
<I/σ(I)>	12.7
Completeness [%]	99.8
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		300	0.1M MES (pH 6.5), 40 % PEG 200