5OU5
Crystal structure of maize chloroplastic photosynthetic NADP(+)-dependent malic enzyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.976254 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 135.961, 147.155, 261.615 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.880 - 2.200 |
R-factor | 0.1623 |
Rwork | 0.160 |
R-free | 0.21056 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.018 |
RMSD bond angle | 1.837 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.880 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.063 | 0.472 |
Number of reflections | 132077 | 12943 |
<I/σ(I)> | 18.67 | 3.51 |
Completeness [%] | 100.0 | 99 |
Redundancy | 5.9 | 6 |
CC(1/2) | 0.999 | 0.906 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.1 M KCl, 0.1 M sodium citrate pH 5.5, 34 % (v/v) pentaerythritol-propylate (5/4 PO/OH) |