5OTW
Extracellular domain of GLP-1 receptor in complex with GLP-1 variant Ala8Hcs/Thr11Cys
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-01 |
| Detector | DECTRIS PILATUS3 R 1M |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.260, 81.200, 42.740 |
| Unit cell angles | 90.00, 98.67, 90.00 |
Refinement procedure
| Resolution | 42.252 - 2.100 |
| R-factor | 0.189 |
| Rwork | 0.184 |
| R-free | 0.23920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zgm |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.723 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2689: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.260 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.107 | 0.980 |
| Rmeas | 1.086 | |
| Rpim | 0.049 | 0.459 |
| Number of reflections | 15304 | |
| <I/σ(I)> | 11.9 | 1.9 |
| Completeness [%] | 98.5 | 95.64 |
| Redundancy | 5.6 | 5.53 |
| CC(1/2) | 0.997 | 0.742 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Morpheus from Molecular Dimensions, solution E12: 0.12 M ethylene Glycols, 0.1 M Buffer system 3 pH 8.5, 12.5% (v/v) MPD, 12.5% (w/v) PEG1000, 12.5% (w/v) PEG3350 |
