5OTU

Extracellular domain of GLP-1 receptor in complex with GLP-1 variant Ala8Hcs/Thr11Hcs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-04
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	39.822, 81.038, 42.945
Unit cell angles	90.00, 97.92, 90.00

Refinement procedure

Resolution	42.535 - 1.800
R-factor	0.1747
Rwork	0.173
R-free	0.21060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zgm
RMSD bond length	0.007
RMSD bond angle	0.840
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2689: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.535	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.045	0.482
Rmeas		0.568
Rpim	0.029	0.298
Number of reflections	24985
<I/σ(I)>	15.6	2.7
Completeness [%]	99.7	99.68
Redundancy	3.4	3.53
CC(1/2)	0.999	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Morpheus from Molecular Dimensions, solution G1: 0.1 M Carboxylic acids, 0.1 Buffer system 1 pH 6.5, 20% (v/v) PEG500mme, 10% (w/v) PEG20000