5OSY

Human Decapping Scavenger enzyme (hDcpS) in complex with m7G(5'S)ppSp(5'S)G mRNA 5' cap analog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918
Spacegroup name	P 21 21 21
Unit cell lengths	57.904, 84.549, 126.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.787 - 2.060
R-factor	0.1806
Rwork	0.178
R-free	0.22660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bl9
RMSD bond length	0.002
RMSD bond angle	0.668
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.160
High resolution limit [Å]	2.060	2.080
Rmerge	0.138	0.764
Number of reflections	38937	6135
<I/σ(I)>	9.84	2.03
Completeness [%]	99.5	99
Redundancy	4.37	4.46
CC(1/2)	0.995	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M Tris-HCl pH 7.6, 31% w/v PEG 4000