5OSI
Structure of retromer VPS29-VPS35C subunits complexed with RidL harpin loop (163-176)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979070 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.554, 138.993, 126.637 |
Unit cell angles | 90.00, 92.78, 90.00 |
Refinement procedure
Resolution | 126.490 - 2.520 |
R-factor | 0.2079 |
Rwork | 0.205 |
R-free | 0.26680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2r17 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.244 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 126.490 | 126.650 | 2.560 |
High resolution limit [Å] | 2.520 | 6.840 | 2.520 |
Rmeas | 0.155 | 0.057 | 1.134 |
Rpim | 0.062 | 0.023 | 0.455 |
Total number of observations | 425544 | ||
Number of reflections | 68126 | 3506 | 3399 |
<I/σ(I)> | 8.5 | 20.3 | 1.9 |
Completeness [%] | 99.8 | 100 | 99.7 |
Redundancy | 6.2 | 6.1 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1 M NaCl, 20% PEG3350 0.1 M Tris pH 8.5 |